SERI-MEE Morphology & Microscopy Core Facilities

The SERI-MEEI Morphology & Microscopy Core provides technical assistance and services for light, confocal, and electron microscopy. Research services include: Routine histological processing, sectioning and staining for frozen, paraffin, methacrylate and epoxy resin tissue preparations. The core facility staff assists with technical problems, procedures, and training on instrumentation inclusive of; Cryostats, automated tissue processors, microtomes, and fluorescence and phase microscopes. The Microscopy Core provides training on two Leica confocal microscopes and Nikon Epifluorescent Microscopes located at SERI-MEE.

State-of-art scanning and transmission electron microscopes and services are available at SERI for MEE faculty and their laboratories. Complete transmission electron microscopy service includes; automated processing of tissue, semi-thin and thin sectioning of cell and tissue samples, TEM imaging and digital image acquisition.

Contact

617-912-0209

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SERI-MEE Morphology Core Services

Researchers should submit all fixed tissue samples in labeled vials and fill out an individual ‘SERI Morphology Work Requisition Form’ for each service located at SERI-MEE Morphology Core Laboratory, room 139.

  1. Paraffin
    Routine = $50.00 per sample (up to 20 slides, H&E stain & unstained slides) After 20 slides: 10 additional slides = +$2.60/sample Special Sectioning (i.e. whole eyes) = $65.00 - $130.00

  2. Methacrylate (i.e. GMA resin)
    Routine = $50.00 per sample (up to 20 slides, H&E stain) After 20 slides: 10 slides = +$2.60/sample Special Sectioning (i.e. whole eyes) = $65.00 - $130.00

  3. Frozen Sectioning
    Routine = $45.00 per sample (using coated slides, up to 20 slides) More than 20 slides = $52.00 per block Special Sectioning (e.g. serial sectioning) = $65.00 - $130.00

  4. Semi-Thin (1-micron) Epoxy
    Routine = $50.00 per sample (up to 2 slides, 1 stain). Includes; Supply of Fixative solution, epoxy resin processing & embedment, semi-thin sectioning and staining (e.g. Toluidine Blue or PPD stain).

  5. Transmission Electron Microscopy (TEM)
    Routine: $210.00 per sample (includes; Supply of Fixative solution, epoxy resin processing & embedment, semi-thin & thin sectioning, and TEM imaging with acquired images on a PC formatted CD-R)

  6. Cryostat Microtome Use:
    No charge for trained users to schedule and use Leica CM1850 Cryostat located in room 139, please contact 
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Some projects may require specialized handling in processing, sectioning and/or special staining. Prices will vary accordingly and should be discussed on an individual basis before work is submitted. If needed, there will be an hourly charge of $65.00 for instructional time.

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